ALIX (PDCD6IP) Mouse Monoclonal Antibody [Clone ID: 3A9]

CAT#: TA352527

Mouse Monoclonal anti-Alix Antibody

Product Images

Western blot analysis of Hela (lane 1), NIH3T3 (lane 2) and Daudi (lane 3) cells using anti-Alix antibody (3A9). GAPDH antibody (poly6314) was used as loading control.

Specifications

Product Data

• Clone Name: 3A9
• Applications: WB
• Recommended Dilution: WB, IP, IF
• Reactivities: Human, Mouse, and Frog
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Recombinant full-length human Alix protein
• Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
• Concentration: lot specific
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 95.8 kDa
• Gene Name: programmed cell death 6 interacting protein
• Database Link:
  NP_037506
  Entrez Gene 18571 MouseEntrez Gene 10015 Human
  UniProt ID Q8WUM4
• Background: Alix (ALG-2-interacting protein X) is an adaptor protein that was first described for its capacity to bind to the calcium-binding protein ALG-2, the expression of which seemed necessary for cell death. The predicted molecular weight is approximately 96 kD. Alix contains an N-terminal Bro1 domain and a C-terminal proline-rich domain (PRD). The PRD contains multiple polyproline motifs, which are potential docking sites for proteins containing SH3 domains, interact with multiple cellular Alix-binding partners that are involved in apoptotic induction, endosomal sorting, and endocytosis. The Bro1 domain interacts with CHMP4b, which is also involved in endosomal sorting.
• Synonyms: AIP1; ALIX; DRIP4; HP95
• Note: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 µg/ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis