Interferon gamma (IFNG) Mouse Monoclonal Antibody [Clone ID: MD-1]

CAT#: TA352730

Mouse Monoclonal Anti-IFN-gamma Antibody

Specifications

Product Data

• Clone Name: MD-1
• Recommended Dilution: ELISA, ELISPOT, Flow cytometry, Immunohistochemistry, Neutralization assay, Western Blot
• Reactivities: Human, Rhesus Macaque
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: IFN-gamma
• Formulation: Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose.
• Concentration: lot specific
• Purification: Ion exchange chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.7 kDa
• Gene Name: interferon, gamma
• Database Link:
  NP_000610
  Entrez Gene 3458 Human
  UniProt ID P01579
• Background: The monoclonal antibody to human IFN-? (clone MD-1) was first described in 1985. It has shown to neutralize the antiviral activity of both natural and recombinant human IFN-? and in a Western Blot the antibody reacts with the 20 kDa and 25 kDa polypeptides present in natural human IFN-? (van der Meide P.H. et al. 1985). The MD-1 antibody has been descriped as useful in a sandwich ELISA and in ELISPOT assays (Meyaard L. et al. 1996, Shen H. et al. 2013, Wassenaar A. et al. 1995 and Wierenga E.A. et al. 1990). Further, the antibody has proven to cross react with chimpanzee and rhesus macaque IFN-? and can be used for immunohistochemical staining of frozen tissues (Jonker M. et al. 2013 and Kap Y. et al. 2009). The MD-1 antibody has been reported for use in intracellular flow cytometric analysis (Hamann D. et al. 1996) and in Luminex systems (Giavedoni L.D. et al. 2005). Other available formats: biotin labeled and FITC labeled.
• Synonyms: IFG; IFI

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Allograft rejection, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Proteasome, Regulation of autophagy, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Type I diabetes mellitus