Ifng Mouse Monoclonal Antibody [Clone ID: DB-12]

CAT#: TA352763

Mouse Monoclonal Anti-IFN-gamma Antibody


USD 645.00

3 Weeks*

Size
    • 500 ug

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Specifications

Product Data
Clone Name DB-12
Recommended Dilution ELISA, ELISPOT, Intracellular staining, Immunohistochemistry, Western Blot
Reactivities Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen IFN-gamma
Formulation Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose.
Concentration lot specific
Purification Ion exchange chromatography.
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 17.9 kDa
Gene Name interferon gamma
Background The mouse anti-rat IFN-? monoclonal antibody (clone DB-12) was first described by Van der Meide P.H. et al. in 1989. The rat IFN-? antibody is of the IgG1 subtype and possesses very low neutralizing activity. In Western blot analysis the DB-12 antibody reacts with the 14 kDa and 18 kDa polypeptides present in rat IFN-? with moderate activity and it has shown not to bind with either mouse IFN-? nor human IFN-?. Further, the DB-12 antibody is highly effective as coating antibody in both sandwich ELISA and ELISPOT assays using biotinylated DB-1 antibody for detection. Also the reverse combination in which the DB-1 antibody functions as coating antibody and biotinylated DB-12 antibody as detector can be used in an ELISA system (Haagmans B.L. et al. 1994). The DB-12 antibody is also applicable in immunohistochemistry on frozen tissues and intracellular staining (Nennesmo, I. et al. 1989 and Bernard, I et al. 1998).
Synonyms IFG; IFI; IFN-gamma
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.