Ifng Mouse Monoclonal Antibody [Clone ID: DB-12]

CAT#: TA352763

Mouse Monoclonal Anti-IFN-gamma Antibody

Specifications

Product Data

• Clone Name: DB-12
• Recommended Dilution: ELISA, ELISPOT, Intracellular staining, Immunohistochemistry, Western Blot
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: IFN-gamma
• Formulation: Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose.
• Concentration: lot specific
• Purification: Ion exchange chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.9 kDa
• Gene Name: interferon gamma
• Database Link:
  NP_620235
  Entrez Gene 25712 Rat
  UniProt ID P01581
• Background: The mouse anti-rat IFN-? monoclonal antibody (clone DB-12) was first described by Van der Meide P.H. et al. in 1989. The rat IFN-? antibody is of the IgG1 subtype and possesses very low neutralizing activity. In Western blot analysis the DB-12 antibody reacts with the 14 kDa and 18 kDa polypeptides present in rat IFN-? with moderate activity and it has shown not to bind with either mouse IFN-? nor human IFN-?. Further, the DB-12 antibody is highly effective as coating antibody in both sandwich ELISA and ELISPOT assays using biotinylated DB-1 antibody for detection. Also the reverse combination in which the DB-1 antibody functions as coating antibody and biotinylated DB-12 antibody as detector can be used in an ELISA system (Haagmans B.L. et al. 1994). The DB-12 antibody is also applicable in immunohistochemistry on frozen tissues and intracellular staining (Nennesmo, I. et al. 1989 and Bernard, I et al. 1998).
• Synonyms: IFG; IFI; IFN-gamma