CHMP4C Rabbit Polyclonal Antibody

CAT#: TA353096L

Rabbit polyclonal anti-Phospho Charged multi vesicular protein 4c antibody

Specifications

Product Data

• Recommended Dilution: WB
• Reactivities: Mammalian
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Phospho Charged multi vesicular protein 4c
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Approx. 30 kDa
• Gene Name: charged multivesicular body protein 4C
• Database Link:
  NP_689497
  Entrez Gene 92421 Human
  UniProt ID Q96CF2
• Background: Borealin interacts directly with Snf7/Shrb/CHMP4 components in both Drosophila and human cells and the two proteins colocalize at the midbody in late cytokinesis. Aurora B phosphorylates CHMP4C at three serine residues located in its C-terminal linker region, a part of the protein known to regulate its ability to form polymers and interact with the membrane. Over-expression of CHMP4C variants mutated in these three residues caused cytokinesis failure, suggesting that Aurora B inhibits CHMP4C activity during cytokinesis. It is proposed that CPC controls abscission timing in both flies and human cells by regulating the function of ESCRT-III Snf7 proteins during cytokinesis through the interaction of its Borealin component with the N-terminus of Shrb/CHMP4 proteins and Aurora B-mediated phosphorylation of the CHMP4C regulatory linker tail.
• Synonyms: Shax3; SNF7-3; VPS32C
• Note: Staining Pattern: CHMP4C is predominantly cytoplasmic and partially colocalizes with Borealin during late cytokinesis, and during abscission CHMP4 is found at the midbody.

Reference Data

• Protein Pathways: Endocytosis