BORIS (CTCFL) Mouse Monoclonal Antibody [Clone ID: 4A7]

CAT#: TA353114L

Mouse monoclonal Anti-BORIS Clone 4A7

Product Images

Agarose gel analyses of PCR products from ChIP assays using mouse anti-BORIS IgM antibody immobilized on Protein L agarose beads. A. PCR products were tested using primers for the known CTS within the human PIM-1 gene. Both BORIS and CTCF shows DNA enrichment at the PIM-1 site. B. PCR perfomed for another characterized CTS (H19ICR). A stronger binding at the H19 site in CTCF ChIPDNA was observed as compared to BORIS ChIP DNA. No antibody reactions for both targets do not show any PCR product. In all ChIP experiments, 1% of sonicated cross-linked cell lysate was used as positive control (Input).

Specifications

Product Data

• Clone Name: 4A7
• Applications: WB
• Recommended Dilution: ChIP ; ELISA ; IF ; IP ; WB
• Reactivities: Human
• Host: Mouse
• Isotype: IgM, kappa
• Clonality: Monoclonal
• Immunogen: The N-terminal domain (aa 2-257) of human BORIS (CTCFL) protein, expressed in E.coli
• Formulation: PBS with 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83
• Gene Name: CCCTC-binding factor like
• Database Link:
  NP_542185
  Entrez Gene 140690 Human
  UniProt ID Q8NI51
• Background: Reagent for research, diagnostic tool. BORIS protein has been identified as Cancer-Testis Antigen (CTA) with testis-specific paralogue of the CCCTC-binding factor. Recent studies have demonstrated that d BORIS is directly responsible for the transcriptional activation of TSP50 (testes-specific protease 50).
• Synonyms: BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
• Note: Protein L beads should be used for ChIP and IP because this antibody is IgM kappa

Reference Data

• Protein Families: Transcription Factors