MDM2 Mouse Monoclonal Antibody [Clone ID: SMP 14]

CAT#: TA353329L

Mouse monoclonal Anti-MDM2 Clone SMP14

Product Images

Western blotting using SMP14 hybridoma supernatant.

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  Strip and reprobe blot with 2A10.

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  Western blotting using SMP14 hybridoma supernatant.

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  Cell staining with SMP14 hybridoma supernatant of MCF7 treated with Nutlin.

Specifications

Product Data

• Clone Name: SMP 14
• Applications: IHC, WB
• Recommended Dilution: ELISA ; IHC ; IF ; IP ; WB
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide CSRPSTSSRRRAISE (residues 154-167 in human MDM2) cross-linked to KLH using glutaldehyde.
• Formulation: PBS with 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: MDM2 proto-oncogene
• Database Link:
  NP_002383
  Entrez Gene 4193 Human
  UniProt ID Q00987
• Background: MDM2 is over-expressed in many tumours. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein.
• Synonyms: ACTFS; hdm2; HDMX
• Note: SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Endocytosis, Glioma, Melanoma, p53 signaling pathway, Pathways in cancer, Prostate cancer, Ubiquitin mediated proteolysis