MET Mouse Monoclonal Antibody [Clone ID: 12.1]

CAT#: TA353331L

Mouse monoclonal Anti-c-Met Clone 12.1

Product Images

Western blotting using HRP-conjugated mAb 12.1. SNU5 cell lysate was used for immunoprecipitation. SC-10 (rabbit polyclonal) is a control.

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  Endogenous c-Met expression in a panel of human gastric cell lines. mAb12.1-HRP recognises 190kD pro-s-Met and 50kD mature c-Met alpha chain. mAb 12.1-HRP does not recognise the 145kD mature c-Met beta-chain.

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  Detection of endogenous c-Met in SNU-5 (c-Met positive) and T47D (c-Met negative) cells by immunofluorescence using purified anti-alpha-chain monoclonal antibodies.

Specifications

Product Data

• Clone Name: 12.1
• Applications: IF, WB
• Recommended Dilution: IHC ; IF ; IP ; WB
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bacterially expressed human c-Met alpha chain
• Formulation: PBS with 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: MET proto-oncogene, receptor tyrosine kinase
• Database Link:
  NP_000236
  Entrez Gene 4233 Human
  UniProt ID P08581
• Background: c-Met is a tyrosine receptor kinase which is activated by its ligand, the hepatocyte growth factor. Activation of c-Met leads to a wide spectrum of biological activities such as motility, angiogenesis, morphogenesis, cell survival and cell regeneration. c-Met is abnormally activated in many tumour types. Aberrant c-Met activation was found to induce tumour development, tumour cell migration and invasion, and the worst and final step in cancer progression, metastasis.
• Synonyms: AUTS9; c-Met; DFNB97; HGFR; RCCP2
• Note: This clone is also referred to as 5E7/G7.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Axon guidance, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma