MMP9 Mouse Monoclonal Antibody [Clone ID: 2C3]

CAT#: TA353338L

Mouse monoclonal Anti-MMP9 Clone 2C3

Product Images

400ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane and detected by immunoblotting with mAb 2C3 specific to MMP-9.

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  IHC staining of human colon carcinoma using 2C3 at a dilution of 1/32.

Specifications

Product Data

• Clone Name: 2C3
• Applications: IHC, WB
• Recommended Dilution: IHC ; IF ; IP ; WB
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Ovalbumin-conjugated synthetic peptide; KLGLGADVAQVT
• Formulation: PBS with 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: matrix metallopeptidase 9
• Database Link:
  NP_004985
  Entrez Gene 4318 Human
  UniProt ID P14780
• Background: Matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes as well as in disease processes. Tissue inhibitors of metalloproteinases (TIMPs) are the main physiological regulators of the MMPs. The TIMPs are secreted proteins that complex with individual MMPs and regulate the activity of specific MMPs. Together, the MMPs and TIMPs form a complex biological system strictly controlling degradation of ECM. The MMPs and TIMPs have a significant role in facilitating tumour invasion and metastasis. Expression of MMP9 has been identified in individual studies as prognostic biomarkers in established and locally advanced colorectal cancer.
• Synonyms: CLG4B; GELB; MANDP2; MMP-9
• Note: IHC: a recommended dilution of 1/32 and pretreatment of antigen retrieval: microwave for 10 minutes at 950W in 0.01M sodium citrate buffer, pH 6.0.

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Bladder cancer, Leukocyte transendothelial migration, Pathways in cancer