NANOG Rabbit Polyclonal Antibody

CAT#: TA353363L

Rabbit polyclonal anti-Nanog antibody

Product Images

This Western blot shows the reactivity of the Nanog antibody at 1/1000 dilution with recombinant (293) or endogenous (ES whole extract, ES nuclear extract). Nanog appears as a double about 37-40 kDa.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ChIP ; FACS ; IF ; IP ; WB
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Nanog
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Nanog homeobox
• Database Link:
  NP_079141
  Entrez Gene 79923 Human
  UniProt ID Q9H9S0
• Background: Nanog is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. Human NANOG protein is a 305 amino acid protein with a conserved homeodomain motif that is localized to the nuclear component of cells. The homeodomain region facilitates DNA binding. Overexpression of Nanog in mouse embryonic stem cells causes them to self-renew in the absence of Leukemia inhibitory factor. In the absence of Nanog, mouse embryonic stem cells differentiate into visceral/parietal endoderm. Loss of Nanog function causes differentiation of mouse embryonic stem cells into other cell types. NANOG overexpression in human embryonic stem cells enables their propagation for multiple passages during which the cells remain pluripotent. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
• Synonyms: homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox
• Note: JT1 and JT2 , same antigen used but from two different rabbits

Reference Data

• Protein Families: Cancer stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Stem cell - Pluripotency