TIMP1 Mouse Monoclonal Antibody [Clone ID: 2A5]

CAT#: TA353510L

Mouse monoclonal Anti-TIMP1 Clone 2A5

Product Images

400ng samples of recombinant human pro-enzyme (lane 1= TIMP-1; lane 2 = TIMP-2) were subjected to SDS-PAGE, transferred to PVDF membrane, and detected with mAb 2A5, specific for TIMP-1.

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  IHC staining of human colon carcinoma using 2A5 at a dilution of 1/8.

Specifications

Product Data

• Clone Name: 2A5
• Applications: IHC, WB
• Recommended Dilution: ELISA ; IHC ; WB
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Ovalbumin-conjugated synthetic peptide; FQALGDAADIR
• Concentration: lot specific
• Purification: Purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: TIMP metallopeptidase inhibitor 1
• Database Link:
  NP_003245
  Entrez Gene 7076 Human
  UniProt ID P01033
• Background: Matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes as well as in disease processes. Tissue inhibitors of metalloproteinases (TIMPs) are the main physiological regulators of the MMPs. The TIMPs are secreted proteins that complex with individual MMPs and regulate the activity of specific MMPs. Together, the MMPs and TIMPs form a complex biological system strictly controlling degradation of ECM. The MMPs and TIMPs have a significant role in facilitating tumour invasion and metastasis. Expression of TIMP1 has been identified in individual studies as prognostic biomarkers in established and locally advanced colorectal cancer.
• Synonyms: CLGI; EPA; EPO; HCI; TIMP; TIMP-1
• Note: IHC: A recommended dilution of 1/8. Recommended pretreatment of antigen retrieval: microwave for 7 minutes at 950W in 0.01M sodium citrate buffer, pH 6.0.

Reference Data

• Protein Families: Druggable Genome, Secreted Protein