Her2 (ERBB2) Mouse Monoclonal Antibody [Clone ID: S-495]

CAT#: TA354161

Mouse anti c-erbB-2/HER-2 Polyclonal Antibody

Product Images

IF: SK-BR-3 cells were fixed and immunoblotted by Mouse anti-Her-2 incubation at a 1:250 dilution.

Specifications

Product Data

• Clone Name: S-495
• Applications: IF
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to the C-terminus of Her2 protein. This sequence is identical in human, mouse, and rat.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~185 kDa
• Gene Name: erb-b2 receptor tyrosine kinase 2
• Database Link:
  NP_001005862
  Entrez Gene 13866 MouseEntrez Gene 24337 RatEntrez Gene 2064 Human
  UniProt ID P04626
• Background: Oncoprotein Her-2/Neu/ c-erbB-2 belongs to one of the four members of the ErbB receptor family. They are transmembrane receptor-like tyrosine kinases which can be autophosphorylated without ligand binding process when it is overexpressed in breast cancers. Her2 is an important biomarker in breast cancer diagnosis. The antibodies/ heregulin can suppress phosphorylation of HER2 on tyrosine Y1248. Endogenous anti-HER2 antibodies can effectively suppress HER2 kinase activity and downstream signaling to inhibit the transformed phenotype of HER2-expressing tumor cells. Thus, anti-Her2 antibodies have been used widely in the treatment of the breast cancer and other carcinomas.
• Synonyms: CD340; HER-2; HER2; MLN 19; NEU; neu; NGL; TKR1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer