gamma Tubulin (TUBG1) Rabbit Polyclonal Antibody

CAT#: TA354181

Rabbit anti Tubulin gamma Polyclonal Antibody

Product Images

IHC: Human colon carcinoma stained with Anti-Tubulin gamma antibody at 1:100 for 30 min, RT. (Staining of formalin-fixed tissues requires boiling tissue section in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: WB 1-5 µg/ml ELISA Not tested IP 2-5 µg/ml IHC 1-2 µg/ml FC Not tested
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to aa 432-451 of human tubulin.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50 kDa
• Gene Name: tubulin gamma 1
• Database Link:
  NP_001061
  Entrez Gene 103733 MouseEntrez Gene 252921 RatEntrez Gene 7283 Human
  UniProt ID P23258
• Background: Tubulin gamma, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). It is an essential structural protein for cell growth organizes microtubule arrays in the nucleus and cytoplasm. Tubulin gamma-depleted cells fail to form functional spindles and arrest during nuclear division. The phosphorylation of Tubulin gamma at Tyr445 plays a regulatory role in microtubule formation. The incidence rate for this phosphorylation event peaks during G1. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity withbeta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental tothe understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulinserve as an essential tool in the detection of the presence and role of this molecule in various cellular settings. Gammatubulin 1 and Gamma tubulin 2 are different isoforms that are 97% homologous.
• Synonyms: CDCBM4; GCP-1; TUBG; TUBGCP1

Reference Data

• Protein Families: Druggable Genome