NF-kB p65 (RELA) Rabbit Polyclonal Antibody

CAT#: TA354228

Rabbit anti NFkB p65 Polyclonal Antibody

Specifications

Product Data

• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Rabbit, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide derived from internal domain of human NF?B p65.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 65 kDa
• Gene Name: RELA proto-oncogene, NF-kB subunit
• Database Link:
  NP_001138610
  Entrez Gene 309165 RatEntrez Gene 5970 Human
  UniProt ID Q04206
• Background: NF-?B (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls the transcription of DNA. NF-?B is important in regulating cellular responses because it belongs to the category of "rapid-acting" primary transcription factors. NF kappa B is a heterodimer consisting of a 50kDa DNA binding subunit and a 65kDa transactivating subunit. In unstimulated cells, the NF-?B dimers are sequestered in the cytoplasm by a family of inhibitors, called I?Bs (Inhibitor of ?B). Activation of the NF-?B is initiated by the signal-induced degradation of I?B proteins. Subsequent to cell stimulation, I?B undergoes phosphorylation, ubiquitination and degradation by a proteosome-dependent pathway, allowing nuclear translocation of the active dimeric NF?B transcription factor. NF-?B complex enters the nucleus where it can 'turn on' the expression of specific genes that have DNA-binding sites for NF-?B nearby. The activation of these genes by NF-?B then leads to the given physiological response, for example, an inflammatory or immune response, a cell survival response, or cellular proliferation.
• Synonyms: NFKB3; p65

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway