CD247 Rabbit Polyclonal Antibody

CAT#: TA354250

Rabbit anti CD3 zeta Polyclonal Antibody

Product Images

WB: Jurkat cell lysate was resolved onto 12% SDSPAGE, and and followed by immunoblotting with Rabbit anti-CD3 zeta antibody at 1:500. Two immunoreactive proteins were observed at ~20 kDa (monomer) and ~42 kDa (Dimmer).

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  IHC: Human tonsil tissue stained with Anti-CD3 zeta antibody at 1:100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the N-terminus of human CD3 zeta. This sequence is identical among human, rat and mouse species.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~20 kDa
• Gene Name: CD247 molecule
• Database Link:
  NP_000725
  Entrez Gene 12503 MouseEntrez Gene 25300 RatEntrez Gene 919 Human
  UniProt ID P20963
• Background: CD3 is a glycoprotein consisting of up to five polypeptide chains (gp20, gp26, p16, p20, p28). It forms three dimmers: a heterodimer of gamma and epsilon chain, a heterodimer of delta and epsilon chains and a homodimer of two zeta chains or a heterodimer of zeta and eta chains. CD3 is required for proper assembly, trafficking and surface expression of TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. CD3 is involved in T cell activation, proliferation and apoptosis pathway. This protein is present on virtually all peripheral blood T cells and thymocytes. The CD3 zeta plays an important role in coupling antigen recognition to several intracellular signal transduction pathway. Low expression of the antigen results in impaired immune response.
• Synonyms: CD3-ZETA; CD3H; CD3Q; CD3Z; IMD25; T3Z; TCRZ

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway