MET Rabbit Polyclonal Antibody

CAT#: TA354334

Rabbit anti c-Met Polyclonal Antibody

Product Images

IHC: The human ovarian carcinoma tissue stained with Rabbit anti-c-Met antibody at 1:50. (Note: formalin-fixed, paraffinembedded sections need 15 minutes heat-induced epitope retrieval in 10 mM citrate buffer, pH 6.0, and 30 minutes incubation at room temperature with the primary antibody.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC Not tested
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide from C-terminus of human c-Met protein
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 155 kDa
• Gene Name: MET proto-oncogene, receptor tyrosine kinase
• Database Link:
  NP_000236
  Entrez Gene 4233 Human
  UniProt ID P08581
• Background: The c-Met oncogene, a member of the tyrosine kinase superfamily, was originally isolated from chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a trans-membrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. The mature c-Met protein is a disulfide-linked heterodimer with Mw 190 kDa composed of a heavily glycosylated domain, a single transmembrane domain and a cytoplasmic tyrosine kinase domain. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
• Synonyms: AUTS9; c-Met; DFNB97; HGFR; RCCP2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Axon guidance, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma