CD68 Mouse Monoclonal Antibody [Clone ID: SPM281]

CAT#: TA354352

Mouse anti Macrophage /CD68 Monoclonal Antibody

Product Images

IHC: Human Tonsil tissue stained with Anti-CD68 antibody, at 1:100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Clone Name: SPM281
• Applications: IHC
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Subcellular fraction of human alveolar macrophages
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 110 kDa
• Gene Name: CD68 molecule
• Database Link:
  NP_001035148
  Entrez Gene 12514 MouseEntrez Gene 287435 RatEntrez Gene 968 Human
  UniProt ID P34810
• Background: CD68 is an intracellular glycoprotein expressed on macrophages and monocytes. CD68 expression has been demonstrated in T cells, NK cells, liver and renal tubules. This antibody is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained.
• Synonyms: GP110; LAMP4; SCARD1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome