c Abl (ABL1) Mouse Monoclonal Antibody [Clone ID: SPM328]

CAT#: TA354360

Mouse anti c-Abl Monoclonal Antibody

Product Images

WB: The whole cell lysate derived from HELA was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Mouse anti–C-Abl antibody at 1:500. An immunoreactive band around 120 kDa was observed.

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  IHC: The human Placenta stained with Mouse anti-c-Abl monoclonal antibody at 1:100. (Note: formalinfixed, paraffin-embedded sections need 15 minutes heatinduced epitope retrieval in 10 mM citrate buffer, pH 6.0, and 30 minutes incubation at room temperature with the primary antibody.

Specifications

Product Data

• Clone Name: SPM328
• Applications: IHC, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: The synthetic peptide corresponding to SH2 domain of c-Abl protein.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~120 kDa
• Gene Name: ABL proto-oncogene 1, non-receptor tyrosine kinase
• Database Link:
  NP_005148
  Entrez Gene 11350 MouseEntrez Gene 25 Human
  UniProt ID P00519
• Background: The c-Abl proto-oncogene encodes a protein tyrosine kinase that is located in the cytoplasm and nucleus. It is activated by certain DNA-damaging agents and regulates induction of the stress-activated c-Jun N-terminal protein kinase (SAPK). In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, the c-Abl proto-oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) As the result of the fusion of c-Abl sequences from chromosome 9 to the Bcr gene on chromosome 22. The molecular consequence of this translocation is the generation of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase.
• Synonyms: ABL; abl; bcr; c-ABL; c-ABL1; JTK7; p150; v-abl

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Axon guidance, Cell cycle, Chronic myeloid leukemia, ErbB signaling pathway, Neurotrophin signaling pathway, Pathogenic Escherichia coli infection, Pathways in cancer, Viral myocarditis