MTA2 Rabbit Polyclonal Antibody

CAT#: TA354430

Rabbit anti PKC (pS345) Polyclonal Antibody

Product Images

WB: The SKBR-3 cells was cultured with 100 uM CDDP for 3 hours, harvest, lysed and ran onto 7.5% SDS-PAGE, transferred onto NC membranes. Then followed by immunoblotting with the Rabbit anti-Phospho-PKC (pS345) at 1:500. An immunoreactive bands was observed around ~90 kDa.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the epitope -EDR-S-KSAP- with a phosphorylated Ser 345 of human PKC.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~90 kDa
• Gene Name: metastasis associated 1 family member 2
• Database Link:
  NP_001317221
  Entrez Gene 23942 MouseEntrez Gene 361724 RatEntrez Gene 9219 Human
  UniProt ID O94776
• Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. With cytotoxic dose (100 uM) of anticancer drug, cisdiamminedlchloroplatinum(ll) (CDDP) incubation, the phosphorylation and translocation into nuclear of PKC in cancer cells including SKBR-3 which express different PKC isoforms was found. Overexpression of PKC was reported in several hemopoietic cell lines and tumors. PKC-epsilon was considered as a full oncogenic potential.
• Synonyms: MTA1L1; PID

Reference Data

• Protein Families: Druggable Genome, Transcription Factors