Estrogen Receptor 1 (ESR1) Rabbit Polyclonal Antibody

CAT#: TA354483

Rabbit anti ER(pS167) Polyclonal Antibody

Product Images

WB: The whole cell lysates derived from MCF-7 were immunoblotted by Rabbit anti-ER (pSer167) at 1:500 (Lane 1). The immunoreactive band is blocked by pre-incubation of immunizing peptide (lane 2)

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  IHC: Human breast cancer tissue stained with Anti-ER (pSer167) antibody, at 1:200 for 10 min at RT. Staining of formalinfixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide surrounding the epitope –LASTN- with a phosphorylation site of Ser167 of human estrogen receptor.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67 kDa
• Gene Name: estrogen receptor 1
• Database Link:
  NP_000116
  Entrez Gene 2099 Human
  UniProt ID P03372
• Background: ER (estrogen receptor) belongs to a member of the steroid receptor superfamily. It contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by its phosphorylation at multiple sites, such as serine 104, 106, 118, 167 and 305. Phosphorylation of certain serine at ER may relate to breast cancer process and on-going treatment.
• Synonyms: ER; Era; ESR; ESRA; ESTRR; NR3A1

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors