Mtor Rabbit Polyclonal Antibody

CAT#: TA354493

Rabbit anti mTOR Polyclonal Antibody

Product Images

WB: The Cell lysate derived from MCF-7 was immuno-precipitated by Rb anti-mTOR, resolved onto 7.5% SDS-PAGe, transferred onto NC membrane, then immune-blotted by the same antibody (Rb anti-mTOR, at 1:500. An immunoreactive band around ~was observed (lane 1). Lane 2 is a negative control.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the C-terminus of human mTOR protein. This sequence is identical among human, rat, mouse, chicken, dog and bovine species.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 290 kDa
• Gene Name: mechanistic target of rapamycin
• Database Link:
  NP_063971
  Entrez Gene 2475 HumanEntrez Gene 56717 MouseEntrez Gene 56718 Rat
  UniProt ID P42346
• Background: The mammalian target of rapamycin (mTOR), also named FKBP12 rapamycin-associated protein (FRAP/RAFT/RAPT/SEP), is a Ser/Thr protein kinase that plays a crucial role in a nutrient-sensitive signalling pathway that regulates cell growth. TOR signalling is potently inhibited by rapamycin, through the direct binding of a FK506-binding protein 12 (FKBP12)/rapamycin complex to the TOR FRB domain, a segment amino terminal to the kinase catalytic domain. mTOR is involved in the regulation of cell growth through initiation of gene translation in response to nutrients such as amino acids (mainly leucine), growth factors, insulin and mitogens. mTOR initiates translation by activating the ribosomal p70S6k protein kinase (S6K1) and by inhibiting the eIF4E inhibitor 4E-BP1. mTOR is phosphorylated at serine 2448 via the phosphatidylinositol 3-kinase (PI3K)/Akt pathway and is autophosphorylated at serine 2481.
• Synonyms: FLJ44809; FRAP; FRAP1; FRAP2; KIAA1303; RAFT1; RAPT1; RAPTOR; RPTOR