CD146 (MCAM) Rabbit Polyclonal Antibody

CAT#: TA354502

Rabbit anti CD146 Polyclonal Antibody

Product Images

WB: The cell lysate derived from Jurkat was immunoblotted by the Rabbit anti-CD146 antibody at 1:500. An immunoreactive band around 100 kDa was observed.

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  IHC: Human Angioscarcoma tissue stained with Rabbit anti-CD146 antibody at 1:50 for 30 min, RT. Staining of formalin-fixed tissues requires boiling tissue section in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant human CD146 protein encoding aa 433-647 expressed in E.coli.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 100 kDa
• Gene Name: melanoma cell adhesion molecule
• Database Link:
  NP_006491
  Entrez Gene 4162 Human
  UniProt ID P43121
• Background: CD146 belongs to the immunoglobulin super-gene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety.
• Synonyms: CD146; MUC18

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane