IRAK4 Mouse Monoclonal Antibody [Clone ID: A8A8]

CAT#: TA354510

Mouse anti IRAK4(pT345) Monoclonal Antibody

Product Images

WB: Western blot of Alu RNA-induced IRAK1 and IRAK4 phosphorylation (1:500) in human RPE cells. Courtesy from Dr. Hirano Cell 149 (4), p847–859, (2012)

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  WB: 7 The 5x10 Jurkat cells were stimulated by 50ng/ml IL-1 for 10min, collected, lysed, resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with Mouse anti-IRAK4 (pT345) antibody at 1:500 (lane 1). Lane 2 was pre-incubated with Phosphospecific peptide, Lane 3 was pre-incubate with nonphospho specific peptide. An immunoreactive band is observed around ~51 kDa.

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  DB: 1 ug peptide was blot onto NC membrane A: IRAK-4 (pT345) B: IRAK-4 (non phosphorylated) Followed by Mouse anti-IRAK-4 (pT345) incubation at a 1:1000 dilution.

Specifications

Product Data

• Clone Name: A8A8
• Applications: Dot, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Bovine, Human, Mouse, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A synthetic peptide surrounding to the epitope -VMTSR- with a phosphorylation site at Thr345 of human IRAK-4 protein. This sequence is identical among human, mouse, bovine and dog.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~51 kDa
• Gene Name: interleukin 1 receptor associated kinase 4
• Database Link:
  NP_001107654
  Entrez Gene 266632 MouseEntrez Gene 486601 DogEntrez Gene 51135 Human
  UniProt ID Q9NWZ3
• Background: IRAK (IL-1 associated serine/threonine kinase) is a critical regulator of Interleukin-1 (IL-1) induced activation of the NFkappaB pathway. IL-1 stimulation leads to the recruitment of interleukin-1 receptor-associated kinase (IRAK) to the IL-1 receptor, where IRAK is phosphorylated, ubiquitinated, and eventually degraded. There are four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). IRAK-4, recently found another IRAK family member necessary for the IL-1 pathway, is able to phosphorylate IRAK in vitro suggests that IRAK-4 might be the IRAK kinase. IRAK4 is required for the efficient recruitment of IRAK to the IL-1 receptor complex.
• Synonyms: IPD1; IRAK-4; NY-REN-64; REN64

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway, Toll-like receptor signaling pathway