Ezrin (EZR) Rabbit Polyclonal Antibody

CAT#: TA354537

Rabbit anti Villin 2/ezrin/p80 Polyclonal Antibody

Product Images

WB: The Cell lysate derived from Hela (lane 2) and HT-29 (Lane 3) were resolved onto 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rb anti-Villin 2 at 1:500. An immunoreactive band around ~80 kDa was observed.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the C-term portion of human Ezrin protein. This sequence is identical to human, rat, mouse, dog, Monkey, zebra fish, and human origins.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~81 kDa
• Gene Name: ezrin
• Database Link:
  NP_001104547
  Entrez Gene 22350 MouseEntrez Gene 54319 RatEntrez Gene 7430 Human
  UniProt ID P15311
• Background: Villin 2, also known as Ezrin, p81, 80K, or cytovillin, is a cytoplasmic peripheral membrane protein which is enriched in microvilli and other cell surface structures. Villin belongs to a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli. Villin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers.
• Synonyms: CVIL; CVL; HEL-S-105; VIL2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton