LRP5 Rabbit Polyclonal Antibody

CAT#: TA354560

Rabbit anti LRP5 (phospho) Polyclonal Antibody

Product Images

WB: The cell lysate derived from conditioned LRP5 transfected HEK293 was immunoprobed by Rabbit anti-LRP5 (pT1492) at 1:500. An immunoreactive band is observed around ~ (1). This band is abolished by pre-incubation with immunizing peptide (2).

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  DB: 1 ug peptide was blot onto NC membrane (A-LRP5 (pT1492); B-LRP5 (Nonphospho); C: Nonrelated Phosphopeptide) was blotted by Rabbit anti-LRP5 (pT1479) a 1:2000

Specifications

Product Data

• Applications: Dot, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the epitope KATLY at a phosphorylation threonine1492 in LRP5 protein
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 175 kDa
• Gene Name: LDL receptor related protein 5
• Database Link:
  NP_001278831
  Entrez Gene 16973 MouseEntrez Gene 293649 RatEntrez Gene 4041 Human
  UniProt ID O75197
• Background: Low-desnsity lipoprotein receptor (LDLR)-related protein 5 (LRP5) is a member of the LDLR family which consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LRP5 cuases several human diseases including bone mineral density variability1, endosteal hyperostosis, autosomal dominant osteopetrosis type I etc. The V171 mutation in LRP5 causes high bone density by impairing the action of a normal antagonist of the Wnt pathway and thus increasing Wnt signaling. The activity of LRP5 and LRP6 can be inhibited by binding of some members of Dickkopf (DKK) family proteins. Upon stimulation with Wnt, LRP5 and LRP6 are phosphorylated at multiple sites including Thr 1479, Ser 1490 and Thr 1492 by kinases such as GSK-3 and CK1.
• Synonyms: BMND1; EVR1; EVR4; HBM; LR3; LRP-5; LRP7; OPPG; OPS; OPTA1; VBCH2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Wnt signaling pathway