IKB alpha (NFKBIA) Rabbit Polyclonal Antibody

CAT#: TA354675

Rabbit anti IkBa (Paired S32/36) (NFKB1A) Polyclonal Antibody

Product Images

WB: The whole cell lysate derived from TNFa-treated Jurkat cells was immunoblotted by Rabbit anti-IkBa (Paired S32/S36) at 1:500. An immune-reactive band is observed around ~42kDa.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the epitope –DSGLDSM- without the dual phosphorylation sites serine 32 and serine 36 of human IkBalpha protein. This sequence is identical within human, mouse, rat, dog and bovine
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~42 kDa
• Gene Name: NFKB inhibitor alpha
• Database Link:
  NP_065390
  Entrez Gene 18035 MouseEntrez Gene 25493 RatEntrez Gene 4792 Human
  UniProt ID P25963
• Background: There are six members in the IkB family, in which IkBa is first protein induced by NF-kB activation. NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as TNF or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome.
• Synonyms: IKBA; MAD-3; NFKBI

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway