LCK Rabbit Polyclonal Antibody

CAT#: TA354687

Rabbit anti p56-LCK (LSK) (pY505) Polyclonal Antibody

Product Images

WB: The whole cell lysate derived from serum starved Jurkat was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti-p56lck (pY505) antibody at 1:500. An immunoreactive band around ~60 kDa is observed.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Bovine, Chicken, Human, Mouse, Rat, Canis
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide derived from Carboxyl-terminus of p56lck protein with a phosphorylation site at Tyr 505 from human, rat, bovine and dog origin.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60 kDa
• Gene Name: LCK proto-oncogene, Src family tyrosine kinase
• Database Link:
  NP_001036236
  Entrez Gene 16818 MouseEntrez Gene 313050 RatEntrez Gene 3932 Human
  UniProt ID P06239
• Background: Lymphocyte specific protein tyrosine kinase (LCK) belongs to Src family protein-tyrosine kinase. It is essential for T-cell development and function. The activity p56lck is regulated by phosphorylation and dephosphorylation of two critical tyrosine residues Tyr394 and Tyr505. Tyr394 and Tyr505 are autophosphorylated after p56lck activation, whereas phosphorylation of Tyr505 is believed to be due to p50csk which negatively modulates P56lck activity. Autophosphorylation at Tyr505 of P56lck may represent an accessory mechanism for the down-regulation of the tyrosine kinase activity of P56lck.
• Synonyms: IMD22; LSK; p56lck; pp58lck; YT16

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Natural killer cell mediated cytotoxicity, Primary immunodeficiency, T cell receptor signaling pathway