Paxillin (PXN) Rabbit Polyclonal Antibody

CAT#: TA354690

Rabbit anti Paxillin Polyclonal Antibody

Product Images

WB: The Hela cell lysate was immunoblotted by Rabbit anti-Paxillin antibody, at 1:250 (Lane 1). Lane 2 was pre-incubated with immunizing peptide.

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  IHC: Human breast cancer tissue stained with Anti-Paxillin antibody at 1:50 for 10 min at RT. Staining of formalinfixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A recombinant protein encoding amino acids 356-558 of human Paxillin.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68 kDa
• Gene Name: paxillin
• Database Link:
  NP_001074324
  Entrez Gene 19303 MouseEntrez Gene 360820 RatEntrez Gene 5829 Human
  UniProt ID P49023
• Background: Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as SRC, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
• Synonyms: FLJ16691

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, VEGF signaling pathway