HIF-1 alpha (HIF1A) Rabbit Polyclonal Antibody

CAT#: TA354706

Rabbit anti HIF, alpha Polyclonal Antibody

Product Images

WB: The tissue lysate derived from hypoxic rat brain was resolved onto 10% SDS-PAGE, transferred onto NC membrane, and immuno-blotted by Rabbit anti-HIF-1 alpha at 1:500. A major immune-reactive band around ~95 kDa was observed.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A twenty-one amino acids synthetic peptide corresponding to the internal sequence of HIF alpha protein. This sequence is identical to human, rat and mouse origins.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~97 kDa
• Gene Name: hypoxia inducible factor 1 alpha subunit
• Database Link:
  NP_001230013
  Entrez Gene 15251 MouseEntrez Gene 29560 RatEntrez Gene 3091 Human
  UniProt ID Q16665
• Background: The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a transcription factor involved in mammalian oxygen homeostasis., HIF-1 is a heterodimer composed of HIF-1 alpha/beta subunits which binds to hypoxia-response elements (HREs) in the promoters of many genes involved in the adaptation to an environment of insufficient oxygen or hypoxia. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation, and increase transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the hypoxic cells. In contrast, HIF-1 beta remains stable under either condition.
• Synonyms: bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma