RHOA Rabbit Polyclonal Antibody

CAT#: TA354715

Rabbit anti Rho(pS188) Polyclonal Antibody

Product Images

WB: The cell lysate derived from conditioned MCF-7 was immunoprobed by Rabbit anti-Rho (pS188) at 1:500. An immunoreactive band is observed around ~ (1). This band is abolished by pre-incubation with immunizing peptide (2).

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  DB: 1 ug peptide was blot onto NC membrane (ARho (pS188); B-Rho (Nonphospho); C: Nonrelated Phosphopeptide) was blotted by Rabbit antiRho (pS188) at a 1:2000 dilution.

Specifications

Product Data

• Applications: Dot, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Chicken, Human, Mouse, Rat, Canis
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the C-term of epitope KKKSG at a phosphorylation Ser188 of human Rho protein
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~24 kDa
• Gene Name: ras homolog family member A
• Database Link:
  NP_001655
  Entrez Gene 11848 MouseEntrez Gene 117273 RatEntrez Gene 387 Human
  UniProt ID P61586
• Background: Ras homolog gene family consists of RhoA, RhoB, RhoC proteins. These proteins interact directly with protein kinase (Rho kinase) to serve as downstream effectors of the activated GTPase. The Rho proteins are involved in the regulation of the actin cytosekeleton. Activation of Rho to the GTP bound state promotes formation of stress fibers and is required for the formation and maintenance of focal adhesions. Pathways stimulated by cytokines, hormones and various forms of stress utilize p21 proteins of the Rho family2. Possible mediators for these responses include protein kinase such as p120PKN, and p150, ROKa/Rho-A kinase, myosin light chain (MLC), phosphatase and PIP 5-kinase.3
• Synonyms: ARH12; ARHA; RHO12; RHOH12

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Neurotrophin signaling pathway, Pathogenic Escherichia coli infection, Pathways in cancer, Regulation of actin cytoskeleton, T cell receptor signaling pathway, TGF-beta signaling pathway, Tight junction, Vascular smooth muscle contraction, Wnt signaling pathway