Neurofibromin (NF1) Rabbit Polyclonal Antibody

CAT#: TA354838

Rabbit anti Neurofibromin(pS2741) Polyclonal Antibody

Product Images

WB: The whole cell lysates derived from hypoxic mouse brain were immunoblotted by Rabbit antiN-F1 (pSer2741) at 1:1000

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  DB: 1 ug peptide was blot onto NC membrane A:NF1 (pS2741) B: NF1 (Nonphospho); C: Non-related phosphospecific PP were blotted at a 1:1000 dilution by: 1: Rabbit anti-NF1 (p2741); 2: Rabbit anti-NF1 (paired 2741)

Specifications

Product Data

• Applications: Dot, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide surrounding the epitope –PTSPY- with a phosphorylation site of Ser2741 of human neurofibromin. This sequence is identical among human, rat, mouse, chicken, bovine and dog.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 240 kDa
• Gene Name: neurofibromin 1
• Database Link:
  NP_000258
  Entrez Gene 4763 Human
  UniProt ID P21359
• Background: The neurofibromatosis type 1 (NF1) tumor suppressor (neurofibromin) is thought to play crucial roles in cellular Ras- and cAMP-dependent kinase (PKA)-associated signals. The absence of or alteration of the neurofibromin protein may lead to neurofibromatosis disease. Recently, a cellular neurofibromin-associating protein, NG,NG-dimethylarginine dimethylaminohydrolase (DDAH)has been identified as a cellular NO/NOS regulator which increases PKA phosphorylation of native neurofibromin in a dose-dependent manner. The PKA accessibility of neurofibromin regulated via DDAH interaction may modulate the cellular function of neurofibromin that is implicated in NF1-related pathogenesis.
• Synonyms: NFNS; VRNF; WSS

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway