Neuropilin 1 (NRP1) Rabbit Polyclonal Antibody

CAT#: TA354848

Rabbit anti Neuropilin-1 (pT916) Polyclonal Antibody

Product Images

WB: The 4 mg protein of whole tissue lysates derived from human brain were immunoprecipitated by 4 ug of Rabbit antiNeuropilin1 (pThr916), and probed by the same antibody at 1:1000. An immunoreactive band at ~130kDa was observed.

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  DB: 1 ug peptide was blot onto NC membrane A:Neuropilin1 (Phosphospecific peptide at pT916) B: Neuropilin1 (Nonphospho PP); C: Non-related phosphospecific PP were blotted at a 1:1000 dilution by: 1: Rabbit anti-Neuropilin1 (pT916); 2: Rabbit anti-Neuropilin1 (paired T916)

Specifications

Product Data

• Applications: Dot, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Bovine, Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide surrounding the epitope –LNTQS- with a phosphorylation site at Thr916. This sequence is identical among human and bovine.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 130 kDa
• Gene Name: neuropilin 1
• Database Link:
  NP_001019799
  Entrez Gene 8829 Human
  UniProt ID O14786
• Background: The neuropilin-1 (Nrp1) is a multifunctional protein, identified principally as a receptor for the class 3 semaphorins and members of the vascular endothelial growth factor (VEGF) family, but it is capable of other interactions. It is a marker of regulatory T cells (Tr), which often carry Nrp1 and latency-associated peptide (LAP)-TGF-beta1 (the latent form). Nrp1 is reported to be expressed in breast cancer cells. The ischemia can increase neuropilin 1 protein expression in experimental rat brain as well. The phosphorylation of Nrp1 is essential for the Nrp1 activation.
• Synonyms: BDCA4; CD304; NP1; NRP; VEGF165R

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Axon guidance