RhoGDI (ARHGDIA) Rabbit Polyclonal Antibody

CAT#: TA354870

Rabbit anti Rho-GDI (Phosphospecific) Polyclonal Antibody

Product Images

WB: The whole cell lysate derived from serum depleted MCF7 (Lane 1) and HELA (Lane 2) were separated in 12% SDS-PAGE and transferred onto NC membrane followed by immuoblotting of Rabbit anti-RhoGDI (pTyr156) at 1:500. An immunoreactive band at ~24 kDa was observed.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide surrounding the epitope – CRPEEYEFL- with a phosphorylation site at Tyrosine 156 of human Rho-GDI alpha protein. This sequence is identical among human, rat, mouse, bovine, chicken and dog.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~23 kDa
• Gene Name: Rho GDP dissociation inhibitor alpha
• Database Link:
  NP_001172006
  Entrez Gene 192662 MouseEntrez Gene 360678 RatEntrez Gene 396 Human
  UniProt ID P52565
• Background: Rho GTPases (Rac, Rho, and Cdc42) play important roles in regulating cell function through their ability to coordinate the actin cytoskeleton, modulate the formation of signaling reactive oxidant species, and control gene transcription. Activation of Rho GTPase signaling pathways requires the regulated release of Rho GTPases from Rho-GDI complexes, followed by their reuptake after membrane cycling. There are two isoforms, Rho-GDI alpha and Rho-GDI beta play synergistic roles in cell migration and development by modulating activation cycle of the Rho proteins. Src kinase binds and phosphorylates RhoGDI both in vitro and in vivo at Tyr156 which causes a dramatic decrease in the ability of RhoGDI to form a complex with RhoA, Rac1, or Cdc42. Src-mediated RhoGDI phosphorylation is a novel physiological mechanism for regulating Rho GTPase cytosol membrane–cycling and activity.
• Synonyms: GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway