LYRIC (MTDH) Rabbit Polyclonal Antibody

CAT#: TA354926

Rabbit anti AEG1(Paired S568) Polyclonal Antibody

Product Images

WB: The cell lysate derived from HELA cells was immuno-blotted by Rabbit anti-Non-phosphospecific AEG1 (Paired S568) at 1:500. An immunoreactive band around 64kDa is observed.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide derived from a non-phosphrylated serine surrounding the epitope -TSWESPKQI-of human AEG1. This sequence is identical to human, mouse, rat and bovine.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~64 kDa
• Gene Name: metadherin
• Database Link:
  NP_848927
  Entrez Gene 67154 MouseEntrez Gene 170910 RatEntrez Gene 92140 Human
  UniProt ID Q86UE4
• Background: AEG1 (Astrocyte elevated gene 1), also called MTDH (Metadherin), or L LYsine-RIch CEACAM1 co-isolated (LYRIC), is 582 aa single transmembrane domain (TMD) protein. It is widely expressed and highly conserved between species. It is recruited during the maturation of the tight junction complex and co-localizes with tight junction proteins ZO-1 and occludin in polarized epithelial cells. AEG-1 is involved in several crucial aspects of tumor progression, including transformation, evasion of apoptosis, invasion, metastasis, and chemoresistance. Overexpression of AEG-1 is frequently observed in melanoma, glioma, neuroblastoma, and carcinomas of breast, prostate, liver, and esophagus and is correlated with poor clinical outcomes. AEG-1 functions as a downstream mediator of the transforming activity of oncogenic Ha-Ras and c-Myc. Furthermore, AEG-1 overexpression activates the PI3K/Akt, nuclear factor ?B (NF?B), and Wnt/β-catenin signaling pathways to stimulate proliferation, invasion, cell survival, and chemoresistance. The lung-homing domain of AEG-1 also mediates the adhesion of tumor cells to the vasculature of distant organs and promotes metastasis. These findings suggest that therapeutic targeting of AEG-1 may simultaneously suppress tumor growth, block metastasis, and enhance the efficacy of chemotherapeutic treatments.
• Synonyms: 3D3; AEG-1; AEG1; LYRIC

Reference Data

• Protein Families: Transmembrane