Cytokeratin 18 (KRT18) Rabbit Polyclonal Antibody

CAT#: TA354929

Rabbit anti CK-18(Non-phospho) Polyclonal Antibody

Product Images

WB: The whole cell lysate derived from A431 was immunoblotted by Rabbit anti-CK18 (Paired 33) antibody at 1:500, an immunoreactive band around ~48kDa is observed.

• 

  IHC: Human breast cancer stained with Anti-Keratin 18 (Paired 33) antibody, at 1:200 for 10 min at RT. Staining of formalinfixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide surrounding to the epitope -SAASVYA- without phosphorylation site at Serine 33 of Cytokeratin 18 protein from human origin. It is identical among human, mouse, rat, etc.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~48 kDa
• Gene Name: keratin 18
• Database Link:
  NP_000215
  Entrez Gene 3875 Human
  UniProt ID P05783
• Background: Keratin polypeptides 8 (basic keratin) and 18 (acidic keratin) exist in combination as K8/K18 which are the cytoskeletal intermediate filament proteins of hepatocytes while K8/K18/K19 are the keratins of hepatobiliary ductal cells. Hepatocyte K8/K18 are highly abundant and behave as stress proteins with injury-inducible expression. K8 and K18 are cytoprotective stress proteins that play a central role in guarding hepatocytes from apoptosis. Hepatocellular carcinoma have been reportedly defined by the use of antibodies that recognize only cytokeratins 8 and 18. K18 Ser33 as an interphase phosphorylation site, which increases its phosphorylation during mitosis in cultured cells and regenerating liver, and as an in vitro cdc2 kinase phosphorylation site.Phosphorylation of Ser33 and Ser52 are involved in keratin subcellular distribution.
• Synonyms: CYK18; K18

Reference Data

• Protein Pathways: Pathogenic Escherichia coli infection