Vinculin (VCL) Rabbit Polyclonal Antibody

CAT#: TA355000

Rabbit Polyclonal Anti-Vinculin Antibody

Product Images

Western blot analysis of Vinculin in A431, Daudi, Jurkat, Raji, and THP-1 cell lysate with Vinculin antibody at 1ug/ml.

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  Immunofluorescence of Vinculin in Jurkat cells with Vinculin antibody at 20ug/ml.

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  Immunocytochemistry of Vinculin in Jurkat cells with Vinculin antibody at 5ug/ml.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 0.5-1µg/ml. IHC starting at 5µg/mL. IF start at 20µg/mL.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Vinculin antibody was raised against a 23 amino acid peptide near the amino of human Vinculin.
• Formulation: Vinculin antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Vinculin antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 120 kDa; Observed: 117 kDa
• Gene Name: vinculin
• Database Link:
  NP_003364
  Entrez Gene 22330 MouseEntrez Gene 305679 RatEntrez Gene 7414 Human
  UniProt ID P18206
• Background: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
• Synonyms: CMD1W; metavinculin; MVCL; vinculin
• Note: Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 µg/ml. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton