B7-2 (CD86) Rabbit Polyclonal Antibody

CAT#: TA355033

Rabbit Polyclonal Anti-CD86 Antibody

Product Images

Western blot analysis of CD86 in (A) Daudi and (B) Raji cell lysate with CD86 antibody at 1ug/ml.

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  Immunohistochemistry of CD86 in human lymph node tissue with CD86 antibody at 2ug/ml.

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  Immunofluorescence of CD86 in human lymph node tissue with CD86 antibody at 20ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1-2µg/mL. IHC: 2µg/mL. Immunoflorescence start at 20µg/mL.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: CD86 antibody was raised against a peptide corresponding to 17 amino acids near the center of human CD86. The immunogen is located within amino acids 160 - 210 of CD86.
• Formulation: CD86 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: CD86 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 36 kDa; Observed: 68 kDa
• Gene Name: CD86 molecule
• Database Link:
  NP_008820
  Entrez Gene 942 Human
  UniProt ID P42081
• Background: CD86, also known as B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD80, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
• Synonyms: B7-2; B70; BU63; CD28LG2; FUN-1; LAB72; MGC34413
• Note: CD86 antibody can be used for Western blot at 1 - 2 µg/mL. Antibody can also be used for Immunohistochemistry at 2 µg/mL. For Immunoflorescence start at 20 µg/mL.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis