B7H3 (CD276) Rabbit Polyclonal Antibody

CAT#: TA355034

Rabbit Polyclonal Anti-CD276 Antibody

Product Images

Western blot analysis of CD276 in (A) human spleen and (B) mouse spleen tissue lysate with CD276 antibody at 0.5ug/ml.

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  Immunohistochemistry of CD276 in human spleen tissue with CD276 antibody at 2ug/ml.

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  Immunofluorescence of CD276 in human spleen tissue with CD276 antibody at 20ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5-1µg/mL.IHC: 2µg/mL. Immunoflorescence start at 20µg/mL.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: CD276 antibody was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of CD276. The immunogen is located within amino acids 484 - 534 of CD276.
• Formulation: CD276 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: CD276 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 35, 43, 57 kDa; Observed: 59 kDa
• Gene Name: CD276 molecule
• Database Link:
  NP_079516
  Entrez Gene 102657 MouseEntrez Gene 315716 RatEntrez Gene 80381 Human
  UniProt ID Q5ZPR3
• Background: CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.
• Synonyms: 4Ig-B7-H3; B7-H3; B7H3
• Note: CD276 antibody can be used for Western blot at 0.5 - 1 µg/mL. Antibody can also be used for Immunohistochemistry at 2 µg/mL. For Immunoflorescence start at 20 µg/mL.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)