GAPDH Rabbit Polyclonal Antibody

CAT#: TA355038

Rabbit Polyclonal Anti-GAPDH Antibody (HRP)

Product Images

Western blot analysis of HRP-GAPDH in 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT4, 3T3, Raji, Ramos, THP-1, U937, human brain, mouse brain, rat brain, rabbit brain, mouse lung, rat lung, mouse liver, rat liver, and chicken small intestine lysate with HRP-GAPDH antibody at 1ug/ml.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 0.5-1µ/ml.
• Reactivities: Chicken, Human, Mouse, Rabbit, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.
• Formulation: HRP-GAPDH antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: HRP-GAPDH antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 37 kDa; Observed: 36 kDa
• Gene Name: glyceraldehyde-3-phosphate dehydrogenase
• Database Link:
  NP_002037
  Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 2597 Human
  UniProt ID P04406
• Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
• Synonyms: G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432
• Note: HRP-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 µ/ml.

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways