GAPDH Mouse Monoclonal Antibody [Clone ID: 12D3D5]

CAT#: TA355044

Mouse Monoclonal Anti-GAPDH Antibody [12D3D5] (biotin)

Product Images

Western blot analysis of GAPDH in multiple cell and tissue lysates with Biotin-GAPDH antibody at 1ug/ml. Lanes 1-20: 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, rabbit brain, mouse lung, mouse liver, and rat liver, respectively.

Specifications

Product Data

• Clone Name: 12D3D5
• Applications: WB
• Recommended Dilution: WB: 1-2µg/ml.
• Reactivities: Human, Mouse, Rabbit, Rat
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Biotin-GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.
• Formulation: Biotin-GAPDH antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Biotin-GAPDH antibody is Protein A purified.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 37 kDa; Observed: 36 kDa
• Gene Name: glyceraldehyde-3-phosphate dehydrogenase
• Database Link:
  NP_002037
  Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 2597 Human
  UniProt ID P04406
• Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
• Synonyms: G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432
• Note: Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 µg/ml.

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways