Vinculin (VCL) Mouse Monoclonal Antibody [Clone ID: 8B5]

CAT#: TA355048

Mouse Monoclonal Anti-Vinculin Antibody [8B5]

Product Images

Western blot analysis of Vinculin in 293, A431, A549, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and human kidney, human breast, mouse brain, mouse lung, mouse spleen, rat heart, rat lung, rat spleen, rat liver, rabbit spleen, rabbit brain and chicken spleen tissue lysate with Vinculin antibody at 1ug/ml.

Specifications

Product Data

• Clone Name: 8B5
• Applications: WB
• Recommended Dilution: WB: 0.5-1µg/ml.
• Reactivities: Chicken, Human, Mouse, Rabbit, Rat
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.
• Formulation: Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Vinculin antibody is purified via Protein A prification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 117 kDa; Observed: 120 kDa
• Gene Name: vinculin
• Database Link:
  NP_003364
  Entrez Gene 22330 MouseEntrez Gene 305679 RatEntrez Gene 7414 Human
  UniProt ID P18206
• Background: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
• Synonyms: CMD1W; metavinculin; MVCL; vinculin
• Note: Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 µg/ml.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton