CTLA4 Mouse Monoclonal Antibody [Clone ID: 8A1]

CAT#: TA355059

Mouse Monoclonal Anti-CTLA-4 Antibody [8A1]

Product Images

Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.5 and 1ug/ml.

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  Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with TA355056, TA355058, and TA355059 antibodies at decreasing concentrations.

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  Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5ug/ml.

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  Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5ug/ml.

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  Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20ug/ml.

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  Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20ug/ml.

• Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20ug/ml.

• Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5ug/ml. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5ug/ml.

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Specifications

Product Data

• Clone Name: 8A1
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: WB: 1-2µg/mL.IHC starting at 5µg/Ml.IF start at 20µg/mL.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.
• Formulation: CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: CTLA-4 Antibody is supplied as protein A purified IgG1.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 25 kDa; Observed: 32 kDa
• Gene Name: cytotoxic T-lymphocyte associated protein 4
• Database Link:
  NP_005205
  Entrez Gene 1493 Human
  UniProt ID P16410
• Background: The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5).
• Synonyms: CD; CD28; CD152; CELIAC3; CTLA-4; GRD4; GSE; ICOS; IDDM12
• Note: CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Autoimmune thyroid disease, Cell adhesion molecules (CAMs), T cell receptor signaling pathway