Lymphocyte Activation Gene 3 (LAG3) Mouse Monoclonal Antibody [Clone ID: 6B12]

CAT#: TA355129

Mouse Monoclonal Anti-LAG3 Antibody [6B12]

Product Images

Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with TA355126, TA355127, TA355128, TA355129, TA355130, TA355131 and TA355132 antibodies at decreasing concentrations.

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  Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 ug/ml.

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  Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.

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  Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 ug/ml.

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  Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 ug/ml.

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  Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 ug/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.

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Specifications

Product Data

• Clone Name: 6B12
• Applications: ELISA, FC, IF, IHC
• Recommended Dilution: ICC start at 1 ug/mL. IF start at 10 ug/mL. IHC start at 5 ug/mL.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: LAG-3 antibody was raised against the extracellular domain of human LAG-3
• Formulation: LAG-3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: LAG-3 Antibody is supplied as protein A purified IgG1.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 55 kDa; Observed: 60, 70, 80 kDa
• Gene Name: lymphocyte activating 3
• Database Link:
  NP_002277
  Entrez Gene 3902 Human
  UniProt ID P18627
• Background: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity ,negatively regulating T-cell function and homeostasis.It is expressed in B, T, and NK cells, monocytes, and dendritic cells,and acts to regulate T cell expansion.LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.
• Synonyms: CD223; FDC; LAG-3

Reference Data

• Protein Families: Transmembrane