S Protein Rabbit Monoclonal Antibody [Clone ID: DM26]

CAT#: TA355177

SARS-CoV-2 RBD rabbit monoclonal antibody, clone DM26

Product Images

Figure 1. Competition flow cytometry assay demonstrating Rabbit anti-RBD monoclonal antibody (clone: DM26) blockade of SARS-CoV-2 (COVID-19) S protein RBD (1µg/ml, TP724025) binding to Expi 293 cell line transfected with human ACE2. IC50=1717ng/ml. The Y-axis represents the geometric mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Specifications

Product Data

• Clone Name: DM26
• Applications: ELISA, FC
• Recommended Dilution: Flow Cyt 1/100
• Reactivities: SARS-CoV-2
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD (Arg 319-Phe541) (TP724025) produced by using human HEK293 cells
• Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
  Preservative: 0.1% Procline 300
• Reconstitution Method: Reconstitute with deionized water
• Purification: Purified from cell culture supernatant by affinity chromatography
• Conjugation: Unconjugated
• Storage: Store at -20°C for 12 months (Avoid repeated freezing and thawing)
• Stability: 12 months from date of despatch
• Predicted Protein Size: 141kDa
• Gene Name: S Protein
• Database Link:
  Entrez Gene 43740568 SARS-CoV-2
• Background: SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.