Snf8 Rabbit Polyclonal Antibody

CAT#: TA355573

Snf8 Antibody - N-terminal region

Product Images

WB Suggested Anti-Snf8 Antibody Titration: 1.0 ug/ml Positive Control: Rat Liver

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  Rabbit Anti-Snf8 Antibody Catalog Number: TA355573 Formalin Fixed Paraffin Embedded Tissue: Human Adult liver Observed Staining: Cytoplasmic Primary Antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 – 2.0 sec Protocol located in Reviews and Data.

Specifications

Product Data

• Applications: IHC, WB
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Expected reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
  Homology: Cow: 100%; Dog: 100%; Goat: 79%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Predicted Protein Size: 28kDa
• Gene Name: SNF8, ESCRT-II complex subunit
• Database Link:
  NP_001007805
  Entrez Gene 287645 Rat
  UniProt ID Q5RK19
• Background: Snf8 is required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is a component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL.
• Synonyms: Dot3; EAP30; hVps22; VPS22