Six1 Rabbit Polyclonal Antibody

CAT#: TA356002

Six1 Antibody - N-terminal region

Product Images

WB Suggested Anti-Six1 Antibody Titration: 1.0 ug/ml Positive Control: Mouse Kidney

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  Rabbit Anti-Six1 Antibody Catalog Number: TA356002 Formalin Fixed Paraffin Embedded Tissue: Human Adult heart Observed Staining: Cytoplasmic,Nuclear (very rare in Nuclear) Primary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 – 2.0 sec Protocol located in Reviews and Data.

Specifications

Product Data

• Applications: IHC, WB
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
  Homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Predicted Protein Size: 32kDa
• Gene Name: sine oculis-related homeobox 1
• Database Link:
  NP_033215
  Entrez Gene 20471 Mouse
  UniProt ID Q62231
• Background: Six1 is a transcription factor that is involved in regulation of organogenesis. It seems to be required for development of kidney, muscle and inner ear and to be involved in later steps of myogenic differentiation. It may be involved in limb tendon and ligament development. It binds a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the myogenin promoter. It is thought to be regulated by association with Dach and Eya proteins. Six1 acts as activator of the IGFBP5 promoter, probably coactivated by EYA2. Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 phosphatase to the SIX1-DACH1 complex. During myogenesis, Six1 seems to act together with EYA2 and DACH2 .
• Synonyms: BOS3; DFNA23; TIP39