SETDB1 Rabbit Polyclonal Antibody

CAT#: TA356011

SETDB1 Antibody - N-terminal region

Product Images

Host: Rabbit Target Name: SETDB1 Sample Type: HT1080 Whole cell lysates Antibody Dilution: 1.0ug/ml

Specifications

Product Data

• Applications: WB
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human SETDB1
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Rabbit, Rat
  Homology: Cow: 92%; Dog: 93%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Rabbit: 85%; Rat: 86%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Predicted Protein Size: 43kDa
• Gene Name: SET domain bifurcated 1
• Database Link:
  Entrez Gene 9869 Human
  UniProt ID Q15047-2
• Background: SETDB1 is a histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
• Synonyms: ESET; H3-K9-HMTase4; KG1T; KIAA0067; KMT1E