UBE3A Rabbit Polyclonal Antibody

CAT#: TA356266

UBE3A Antibody - middle region

Product Images

WB Suggested Anti-UBE3A Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: Hela cell lysateUBE3A is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells

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  Lanes: Lane 1: 7ug HeLa lysate+EGFP SiRNA Lane 2: 7ug HeLa lysate+UBE3A SiRNA Primary Antibody Dilution: 1:300 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:500 Gene Name: UBE3A Submitted by: Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto University

Specifications

Product Data

• Applications: WB
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE3A
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
  Homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Predicted Protein Size: 101kDa
• Gene Name: ubiquitin protein ligase E3A
• Database Link:
  NP_000453
  Entrez Gene 22215 MouseEntrez Gene 7337 Human
  UniProt ID Q05086
• Background: UBE3A is an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
• Synonyms: ANCR; AS; E6-AP; E6AP; EPVE6AP; FLJ26981; HPVE6A

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis