HLA II Monoclonal Antibody [Clone ID: HKB1Biotin]

CAT#: TA363835

HLA ll (human leukocyte antigen class ll) mouse anti human, clone HKB1 Biotin

Product Images

TA363835, Clone HKB1 Biotin, human tonsil, frozen section

Specifications

Product Data

• Clone Name: HKB1Biotin
• Applications: IHC
• Recommended Dilution: Tested for immunohistochemistry (IHC); has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 0.5-1µg/ml (1:400-1:800) Paraffin sections: not tested Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil
• Reactivities: Human
• Clonality: Monoclonal
• Immunogen: REH cells, a human B cell precursor leukemia cell line.
• Specificity: Human: HLA II Other: not tested
  
  Epitope: The antigen is HLA II, the epitope has not been further characterized.
• Formulation: Affinity purified, liquid Supplied as a 0.5ml solution. This stock solution contains 0.4mg/ml IgM, phosphate buffered saline pH 7.2 (PBS) and 0.05% (v/v) Kathon CG as a preservative.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
• Background: Monoclonal antibody HKB1 recognizes all HLA class II antigens, the 3 major (HLA-DP, -DQ and -DR) and 2 minor Major Histocompatibility Complex MHC class II proteins (HLA-DM and -DO). The genes of the class II combine to form heterodimeric (αβ) protein receptors that are typically expressed on the surface of antigen presenting cells (APCs): dendritic cells B-cells and macrophages. HLA class II is presented on activated T-cells. HLA class II molecules present exogenously derived antigen to the T cell receptor (TCR) on CD4+ T lymphocytes.