CD69 Monoclonal Antibody [Clone ID: FN50 Biotin]

CAT#: TA363838

CD69 (early T-cell activation antigen p60, Leu-23), mouse anti human, clone FN50 Biotin

Product Images

TA363838, Clone FN5O Biotin, human tonsil, frozen section

Specifications

Product Data

• Clone Name: FN50 Biotin
• Applications: IHC
• Recommended Dilution: Tested for immunohistochemistry (IHC); has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 2 - 4µg/ml (1:50 - 1:100) Paraffin sections: does not react on routinely processed paraffin sections. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil
• Reactivities: Human
• Clonality: Monoclonal
• Specificity: Human: CD69 Other: not tested.
  
  Epitope: The antigen is CD69. The epitope is a 53kD molecule. Under reducing conditions it showed two bands of 29 and 32 kD.
  
  Distribution: Isolated cells: > 90% of activated human peripheral blood lymphocytes. Tissue sections: In tonsil the antigen is strongly expressed on CD4+ T-cells in the light zone of the follicle centre, on extrafollicular T-cells, and weakly on mantle zone B-cells. In the spleen, the antigen is found on CD4+ T-cells in the middle of the marginal zone, while in thymus most of the medullary thymocytes are strongly positive.
• Formulation: Affinity purified, biotinylated, lyophilized Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA), and 0.05% (v/v) Kathon CG as a preservative.
• Concentration: N/A
• Conjugation: Biotin
• Storage: Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
• Gene Name: CD69 molecule
• Database Link:
  Entrez Gene 969 Human
  UniProt ID Q07108
• Synonyms: AIM; BL-AC/P26; CLEC2C; EA1; GP32/28; Leu-23; MLR-3