Spn Mouse Monoclonal Antibody [Clone ID: WEN3]

CAT#: TA363851

CD43 (leukosialin, sialophorin), mouse anti rat, clone WEN3

Product Images

TA363851, Clone WEN3, rat spleen, paraffin section

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  TA363851, Clone WEN3, rat spleen, paraffin section

Specifications

Product Data

• Clone Name: WEN3
• Applications: IHC
• Recommended Dilution: Each lot of this antibody has been tested and validated for immunohistochemistry (IHC); the antibody has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 0.1µg/ml (1:4000) Paraffin sections: 5µg/ml (1:80); microwave pretreatment for antigen retrieval is recommended. Optimal dilutions should be determined by the end user. Suggested positive control: rat spleen.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: rat lymphokine activated killer (LAK) cells
• Specificity: Rat: subpopulations of CD4+, CD8+ T-cells. Other: does not react with porcine tissue.
  
  Epitope: The antigen has been identified as rat CD43, the epitope has not been characterized.
  
  Distribution: Tissue sections: Cells in T-cell areas mostly show a weak reaction, whereas strong staining is seen in scattered splenic cells in the red pulp.
• Formulation: Affinity purified from cell culture supernatant, lyophilized, packaged under nitrogen. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.4mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: 1 year at 4° - 8°C. Minimize repeated thawing and freezing of the stock solution.
• Gene Name: sialophorin
• Database Link:
  Entrez Gene 24796 Rat
  UniProt ID P13838
• Background: Monoclonal antibody WEN3 reacts with rat CD43, also known as Leukosialin, Sialophorin or W3/13 antigen. CD43 is a major glycoprotein of thymocytes and T lymphocytes, a factor in lectin binding and counter-receptor for Siglec-1 (Sialoadhesin). CD43 is a single-pass type I membrane protein with a 371 amino acid backbone that presents carbohydrate ligands to selectins. T cells express two glycoforms of CD43, with molecular weights of 115 kDa and 130 kDa due to different glycosylation patterns. This antibody was produced in vitro from cell culture supernatant.
• Synonyms: CD43; Galactoglycoprotein; GALGP; GPL115; leukosialin; LSN; Sialophorin